1DOA

Structure of the rho family gtp-binding protein cdc42 in complex with the multifunctional regulator rhogdi

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F2
Synchrotron site	CHESS
Beamline	F2
Temperature [K]	100
Detector technology	CCD
Collection date	1999-04-15
Detector	ADSC QUANTUM 4
Spacegroup name	H 3
Unit cell lengths	83.940, 83.940, 191.160
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.900 - 2.600
R-factor	0.257
Rwork	0.257
R-free	0.32000
RMSD bond length	0.007
RMSD bond angle	1.640 *
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.900 *
High resolution limit [Å]	2.600 *	2.600 *
Rmerge	0.057 *	0.372 *
Total number of observations	90533 *
Number of reflections	15454 *
Completeness [%]	100.0 *	99.8 *
Redundancy	5.9 *	2.4 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	291	15% PEG 4000, 100 mM Glutamic Acid, 25 mM MgSO4, 50 mM Tris, 2 mM NaN3, 2 mM LDAO , pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 18K, temperature 291K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	reservoir	PEG4000	15-20 (%)
3	1	reservoir		25 (mM)
4	1	reservoir	glutamic acid	100 (mM)
5	1	reservoir	lauryldimethylamine-oxide	2 (mM)
6	1	reservoir	Tris-HCl	100 (mM)