1DNM
CRYSTAL STRUCTURE AND SEQUENCE-DEPENDENT CONFORMATION OF THE A.G MIS-PAIRED OLIGONUCLEOTIDE D(CGCAAGCTGGCG)
Experimental procedure
| Source type | SEALED TUBE |
| Temperature [K] | 280 |
| Detector technology | DIFFRACTOMETER |
| Detector | ENRAF-NONIUS FAST |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 25.290, 41.780, 64.760 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | ? - 2.500 |
| R-factor | 0.193 |
| Refinement software | NUCLSQ |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.500 |
| Rmerge | 0.119 |
| Total number of observations | 2495 * |
| Number of reflections | 1710 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | other * | 7.2 | 5 * | pH 7.20, VAPOR DIFFUSION, temperature 277.00K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | MGCL2 | ||
| 4 | 1 | 1 | SPERMINE | ||
| 5 | 1 | 2 | WATER | ||
| 6 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | dodecamer | 0.75 (mM) | |
| 2 | 1 | drop | 25 (mM) | ||
| 3 | 1 | drop | spermine | 2.5 (mM) | |
| 4 | 1 | drop | sodium cacodylate | 30 (mM) | |
| 5 | 1 | reservoir | MPD | 32 (%(v/w)) |
