1DN5
SOLVATION OF THE LEFT-HANDED HEXAMER D(5BRC-G-5BRC-G-5BRC-G) IN CRYSTALS GROWN AT TWO TEMPERATURES
Experimental procedure
| Temperature [K] | 310 |
| Detector technology | DIFFRACTOMETER |
| Detector | ENRAF-NONIUS CAD4 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 17.930, 30.830, 44.730 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.400 |
| R-factor | 0.1247 |
| Refinement software | NUCLSQ |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 10.000 |
| High resolution limit [Å] | 1.400 |
| Number of reflections | 3765 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 * | 37 * | VAPOR DIFFUSION, temperature 310.00K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | NA CACODYLATE | ||
| 4 | 1 | 1 | NACL | ||
| 5 | 1 | 2 | WATER | ||
| 6 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | sodium cacodylate | 20 (mM) | |
| 2 | 1 | drop | 200 (mM) | ||
| 3 | 1 | drop | protein | 1 (mg/ml) | |
| 4 | 1 | reservoir | MPD | 10-60 (%) |
