1DMW

CRYSTAL STRUCTURE OF DOUBLE TRUNCATED HUMAN PHENYLALANINE HYDROXYLASE WITH BOUND 7,8-DIHYDRO-L-BIOPTERIN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1999-07-03
Detector	MARRESEARCH
Spacegroup name	C 2 2 21
Unit cell lengths	66.357, 107.991, 124.591
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.000
R-factor	0.2 *
Rwork	0.200
R-free	0.24590
RMSD bond length	0.015
RMSD bond angle	22.100 *
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.072	0.311
Total number of observations	102504 *
Number of reflections	29570	2093 *
<I/σ(I)>	11.4
Completeness [%]	96.5	69.2
Redundancy	3.5	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.8	277	PEG2000, NaCl, PIPES, Ethylene Glycol, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	ethylene glycol	10 (%(v/v))	cryoprotectant
2	1	drop	7,8-dihydro-L-biopterin	1 (mM)
3	1	reservoir	PEG2000	20-30 (%(w/v))
4	1	reservoir	PIPES	40-80 (mM)	pH6.8
5	1	drop	protain	4.5 (mg/ml)