1DM5

ANNEXIN XII E105K HOMOHEXAMER CRYSTAL STRUCTURE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1997-05-14
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	68.943, 162.544, 188.798
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 1.930
R-factor	0.208 *
Rwork	0.208
R-free	0.26300
RMSD bond length	0.009
RMSD bond angle	1.500
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	1.930
High resolution limit [Å]	1.900	1.900
Rmerge	0.056	0.295
Total number of observations	969367 *
Number of reflections	156877
<I/σ(I)>	20.6
Completeness [%]	93.5	93.8 *
Redundancy	6.18

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	277	0.2 M AMMONIUM ACETATE 0.1 M SODIUM CITRATE PH 5.6 80 % 2-METHYL-PENTANEDIOL, VAPOR DIFFUSION, HANGING DROP, temperature 4K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	8 (mg/ml)
2	1	reservoir	ammonium acetate	0.2 (M)
3	1	reservoir	sodium citrate	0.1 (M)
4	1	reservoir	2-methylpentanediol	80 (%)