1DLV
BIOSYNTHETIC THIOLASE FROM ZOOGLOEA RAMIGERA IN COMPLEX WITH COA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | ENRAF-NONIUS |
Temperature [K] | 100 |
Detector technology | AREA DETECTOR |
Collection date | 1999-02-15 |
Detector | MARRESEARCH |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 84.818, 79.302, 149.979 |
Unit cell angles | 90.00, 93.49, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.290 |
R-factor | 0.242 * |
Rwork | 0.242 |
R-free | 0.29300 |
RMSD bond length | 0.019 * |
RMSD bond angle | 2.600 * |
Data scaling software | XDS |
Phasing software | AMoRE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.450 |
High resolution limit [Å] | 2.290 | 2.290 |
Rmerge | 0.127 | 0.274 |
Number of reflections | 86956 | |
<I/σ(I)> | 13.7 | |
Completeness [%] | 93.0 | 95.7 |
Redundancy | 4.3 | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 294 | AMMONIUM SULPHATE, LITHIUM SULPHATE, SODIUM ACETATE, pH 5, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | ammonium sulfate | 0.9 (M) | |
2 | 1 | reservoir | lithium sulfate | 1 (M) | |
3 | 1 | reservoir | sodium acetate | 0.1 (M) | |
4 | 1 | reservoir | dithiothreitol | 1 (mM) | |
5 | 1 | reservoir | sodium azide | 1 (mM) | |
6 | 1 | reservoir | EDTA | 1 (mM) |