1DLO

HUMAN IMMUNODEFICIENCY VIRUS TYPE 1

Experimental procedure

Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Detector technology	IMAGE PLATE AREA DETECTOR
Collection date	1994-10
Spacegroup name	C 1 2 1
Unit cell lengths	235.500, 70.300, 93.300
Unit cell angles	90.00, 106.10, 90.00

Refinement procedure

Resolution	8.000 - 2.700
R-factor	0.249
Rwork	0.249
R-free	0.33600
RMSD bond length	0.010 *
RMSD bond angle	2.000
Data reduction software	DENZO
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
High resolution limit [Å]	2.700 *
Rmerge	0.055
Total number of observations	142770 *
Number of reflections	41049
Completeness [%]	99.5
Redundancy	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7 *		Kohlstaedt, L.A., (1992) Science, 256, 1783. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	RT	6-8 (mg/ml)
10	1	reservoir	beta-octylglucoside	0.2 (%(w/v))
11	1	reservoir	glycerol	10 (%(v/v))
12	1	reservoir	PEG8000	14 (%(w/v))
13	1	reservoir	sodium azide	0.02 (%)
2	1	drop	Bis-Tris propane	25 (mM)
3	1	drop		50 (mM)
4	1	drop	beta-octylglucoside	0.1 (%(w/v))
5	1	drop	glycerol	5 (%(v/v))
6	1	drop	PEG8000	7 (%(w/v))
7	1	drop	sodium azide	0.01 (%)
8	1	reservoir	Bis-Tris propane	50 (mM)
9	1	reservoir		100 (mM)