1DL2
CRYSTAL STRUCTURE OF CLASS I ALPHA-1,2-MANNOSIDASE FROM SACCHAROMYCES CEREVISIAE AT 1.54 ANGSTROM RESOLUTION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1998-11-05 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 88.397, 88.397, 153.220 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 1.540 |
| R-factor | 0.209 |
| Rwork | 0.209 |
| R-free | 0.22700 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 21.400 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.640 |
| High resolution limit [Å] | 1.540 | 1.540 |
| Rmerge | 0.060 | 0.440 |
| Total number of observations | 649016 * | |
| Number of reflections | 100985 | |
| <I/σ(I)> | 12.5 | |
| Completeness [%] | 99.5 | 99.5 |
| Redundancy | 6.5 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 5.6 | 293 | 0.1 M NA CITRATE, 0.25 M AMMONIUM ACETATE, 17% PEG2000 MME, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | mPEG2000 | 17-19 (%(w/v)) | |
| 3 | 1 | reservoir | sodium citrate | 100 (mM) | |
| 4 | 1 | reservoir | ammonium acetate | 250 (mM) |
