1DKG
CRYSTAL STRUCTURE OF THE NUCLEOTIDE EXCHANGE FACTOR GRPE BOUND TO THE ATPASE DOMAIN OF THE MOLECULAR CHAPERONE DNAK
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1996-10 |
| Spacegroup name | P 41 |
| Unit cell lengths | 149.430, 149.430, 49.030 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.800 |
| R-factor | 0.223 |
| Rwork | 0.223 |
| R-free | 0.31700 |
| Structure solution method | MIR |
| RMSD bond length | 0.012 * |
| RMSD bond angle | 1.580 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.851) |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.093 * | 0.179 * |
| Total number of observations | 754420 * | |
| Number of reflections | 26024 | |
| <I/σ(I)> | 18 | 7 |
| Completeness [%] | 95.7 | 86.4 |
| Redundancy | 29 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 4.6 | 4 * | pH 4.6 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 40 (mg/ml) | |
| 2 | 1 | reservoir | PEG8000 | 3.5 (%) | |
| 3 | 1 | reservoir | ethylene glycol | 15 (%) | |
| 4 | 1 | reservoir | sodium acetate | 50 (mM) | |
| 5 | 1 | reservoir | lithium sulfate | 190 (mM) | |
| 6 | 1 | reservoir | dithiothreitol | 5-10 (mM) | |
| 7 | 1 | reservoir | (D,L)-methionine | 10 (mM) |
