1DJY

PHOSPHOINOSITIDE-SPECIFIC PHOSPHOLIPASE C-DELTA1 FROM RAT COMPLEXED WITH INOSITOL-2,4,5-TRISPHOSPHATE

Experimental procedure

Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID2
Synchrotron site	ESRF
Beamline	ID2
Detector technology	IMAGE PLATE
Collection date	1995-03-28
Detector	MARRESEARCH
Spacegroup name	F 41 3 2
Unit cell lengths	397.130, 397.130, 397.130
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.800
R-factor	0.2 *
Rwork	0.210
R-free	0.26000
Structure solution method	DIFFERENCE FOURIER
Starting model (for MR)	1ISD
RMSD bond length	0.019
RMSD bond angle	18.476 *
Data reduction software	MOSFLM
Phasing software	CCP4
Refinement software	TNT (5E)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.000 *
High resolution limit [Å]	2.800 *
Rmerge	0.094	0.364 *
Total number of observations	396081 *
Number of reflections	59742
Completeness [%]	90.9	91.4 *
Redundancy	6.6	4.6 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *		12 *	used to seeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	CHAPSO	0.1 (%(w/v))
3	1	drop	ammonium phosphate	2.0 (M)
4	1	drop	dithiothreitol	1 (mM)
5	1	reservoir	ammonium phosphate	1.45 (M)
6	1	reservoir	dithiothreitol	1 (mM)