1DJE
CRYSTAL STRUCTURE OF THE PLP-BOUND FORM OF 8-AMINO-7-OXONANOATE SYNTHASE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, Hamburg |
Beamline | X11 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1998-10-01 |
Detector | MARRESEARCH |
Spacegroup name | P 31 1 2 |
Unit cell lengths | 58.870, 58.870, 200.800 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 10.000 - 1.710 |
R-factor | 0.186 |
Rwork | 0.186 |
R-free | 0.24500 |
Structure solution method | AB INITIO |
RMSD bond length | 0.009 |
RMSD bond angle | 0.120 |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 51.000 | 1.690 |
High resolution limit [Å] | 1.670 | 1.670 |
Rmerge | 0.080 | 0.608 |
Number of reflections | 47168 | |
<I/σ(I)> | 16.7 | |
Completeness [%] | 100.0 | 99.9 |
Redundancy | 4.9 | 5.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 289 | AMMONIUM SULPHATE, SODIUN CHLORIDE, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | drop | bis-Tris/propane | 10 (mM) | |
3 | 1 | drop | 50-100 (mM) | ||
4 | 1 | reservoir | ammonium sulfate | 1.6 (M) | |
5 | 1 | reservoir | bis-Tris/propane | 200 (mM) | |
6 | 1 | reservoir | glycerol | 30 (%(v/v)) |