1DIX

CRYSTAL STRUCTURE OF RNASE LE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1998-07-31
Detector	RIGAKU RAXIS IIC
Spacegroup name	P 21 21 21
Unit cell lengths	74.020, 78.790, 32.930
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 1.650
R-factor	0.219 *
Rwork	0.219
R-free	0.27800
Structure solution method	MIR
RMSD bond length	0.020
RMSD bond angle	0.039
Phasing software	MLPHARE
Refinement software	REFMAC

Data quality characteristics

	Overall
Low resolution limit [Å]	100.000
High resolution limit [Å]	1.650
Rmerge	0.054 *
Total number of observations	144121 *
Number of reflections	22385
Completeness [%]	93.4
Redundancy	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	293	PEG1540, CITRATE, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	drop	citrate	20 (mM)
3	1	reservoir	PEG1540	36 (%(w/v))
4	1	reservoir	citrate	0.1 (M)