1DIK
PYRUVATE PHOSPHATE DIKINASE
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 295 |
| Detector technology | AREA DETECTOR |
| Collection date | 1991-01-07 |
| Detector | SIEMENS |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 89.800, 58.800, 102.000 |
| Unit cell angles | 90.00, 94.80, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.300 |
| R-factor | 0.182 |
| Rwork | 0.182 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.100 |
| Data reduction software | XENGEN |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 28.300 |
| High resolution limit [Å] | 2.300 |
| Rmerge | 0.082 |
| Number of reflections | 44378 |
| Completeness [%] | 93.0 |
| Redundancy | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 | 30 * | pH 7.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | imidazole | 20 (mM) | |
| 3 | 1 | drop | 100 (mM) | ||
| 4 | 1 | drop | EDTA | 0.1 (mM) | |
| 5 | 1 | drop | 2-mercaptoethanol | 1 (mM) | |
| 6 | 1 | reservoir | ammonium sulfate | 50-55 (%sat) |
