1DHR

CRYSTAL STRUCTURE OF RAT LIVER DIHYDROPTERIDINE REDUCTASE

Experimental procedure

Spacegroup name	C 2 2 21
Unit cell lengths	50.100, 139.130, 64.930
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 * - 2.300
R-factor	0.154 *
RMSD bond length	0.010
RMSD bond angle	2.300
Phasing software	X-PLOR
Refinement software	PROLSQ

Data quality characteristics

	Overall
Low resolution limit [Å]	8.000 *
High resolution limit [Å]	2.300 *
Rmerge	0.060 *
Number of reflections	10097 *
Completeness [%]	96.3 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.8 *	4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein NADH solution	10 (mg/ml)	0.005ml
2	1	drop	PEG4500	17 (%(w/v))
3	1	drop	ethanol	11 (%(v/v))
4	1	drop	Tris-HCl	0.05 (M)
5	1	reservoir	PEG4500	17 (%(w/v))
6	1	reservoir	ethanol	11 (%(v/v))
7	1	reservoir	Tris-HCl	0.05 (M)