1DHP
DIHYDRODIPICOLINATE SYNTHASE
Experimental procedure
Detector technology | IMAGE PLATE |
Detector | MARRESEARCH |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 122.410, 122.410, 111.220 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 8.000 - 2.300 |
R-factor | 0.197 |
Rwork | 0.197 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.662 |
Data reduction software | MOSFLM |
Phasing software | X-PLOR |
Refinement software | X-PLOR |
Data quality characteristics
Overall | |
Rmerge | 0.038 |
Redundancy | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 4 * | Laber, B., (1992) Biochem.J., 288, 691. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | ammonium salfate | 1.25-3.0 (M) | |
2 | 1 | drop | sodium potassium phosphate | 1.25-3.0 (M) | |
3 | 1 | drop | sodium citrate | 0.8-1.6 (M) | |
4 | 1 | drop | phosphate | 0.2-1.5 (M) | |
5 | 1 | reservoir | PEG6000 | 10 (%(w/v)) |