1DGF

HUMAN ERYTHROCYTE CATALASE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	200
Detector technology	IMAGE PLATE
Collection date	1999-03-06
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	83.717, 142.505, 232.066
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.500
R-factor	0.174
Rwork	0.172
R-free	0.19200
RMSD bond length	0.007 *
RMSD bond angle	0.007
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.550
High resolution limit [Å]	1.500	1.500
Rmerge	0.045 *	0.279 *
Total number of observations	1131638 *
Number of reflections	401102
<I/σ(I)>	20
Completeness [%]	90.6	82.2
Redundancy	2.8	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	298	6.5 - 8.0% PEG4000, PROTEIN AT 40 MG/ML IN 50MM TRISCL, PH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	40 (mg/ml)
2	1	drop	Tris-HCl	50 (mM)
3	1	reservoir	PEG4000	6.5-8.0 (%)