1DFV
CRYSTAL STRUCTURE OF HUMAN NEUTROPHIL GELATINASE ASSOCIATED LIPOCALIN MONOMER
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1998-08-09 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 115.021, 115.021, 117.638 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.600 |
R-factor | 0.281 |
Rwork | 0.281 |
R-free | 0.29300 |
RMSD bond length | 0.008 |
RMSD bond angle | 28.100 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | EPMR |
Refinement software | CNS (0.5) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.520 |
High resolution limit [Å] | 2.480 | 2.480 |
Rmerge | 0.052 | 0.255 |
Number of reflections | 25147 | |
<I/σ(I)> | 43.8 | |
Completeness [%] | 87.9 | 60 |
Redundancy | 4.2 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 7 | 18 * | PEG 8k, Ammonium Sulfate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | reservoir | PEG8000 | 21-23 (%(w/w)) | |
3 | 1 | reservoir | ammonium sulfate | 12-14 (%sat) | |
4 | 1 | reservoir | glycerol | 10 (%(v/v)) | |
5 | 1 | reservoir | PIPES | 25 (mM) | |
6 | 1 | reservoir | dithiothreitol | 10 (mM) |