1DFJ

RIBONUCLEASE INHIBITOR COMPLEXED WITH RIBONUCLEASE A

Experimental procedure

Detector technology	IMAGE PLATE
Collection date	1994
Detector	RIGAKU RAXIS II
Spacegroup name	I 4
Unit cell lengths	133.300, 133.300, 86.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 2.500
R-factor	0.194
Rwork	0.194
RMSD bond length	0.013
RMSD bond angle	1.826
Data reduction software	DENZO
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	40.000 *
High resolution limit [Å]	2.500 *
Rmerge	0.100
Total number of observations	111046 *
Number of reflections	18859
Completeness [%]	71.4
Redundancy	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	7 *	21 *	Kobe, B., (1994) J.Mol.Biol., 241, 288. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium salfate	1.3 (M)
2	1	reservoir	sodium acetate	100 (mM)
3	1	reservoir	DTT	20 (mM)
4	1	drop	RI	22 (mg/ml)
5	1	drop	potassium phosphate	16 (mM)
6	1	drop	DTT	16 (mM)
7	1	drop	RNase A	5.6 (mg/ml)