1DF8
S45A MUTANT OF STREPTAVIDIN IN COMPLEX WITH BIOTIN
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 127 |
Detector technology | IMAGE PLATE |
Collection date | 1998-10-23 |
Detector | RIGAKU |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 46.200, 93.900, 104.600 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 1.510 |
R-factor | 0.165 * |
Rwork | 0.169 |
R-free | 0.21700 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1swa |
RMSD bond length | 0.009 * |
RMSD bond angle | 0.029 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 10.000 | 1.530 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.052 | 0.103 |
Total number of observations | 167399 * | |
Number of reflections | 30427 | |
<I/σ(I)> | 2.15 | |
Completeness [%] | 82.4 | 4 |
Redundancy | 5.5 | 1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | unknown * | 4.5 | 294 | 40% SATURATED AMMONIUM SULFATE, 0.1 M SODIUM ACETATE, pH 4.5, temperature 294.K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | ammonium sulfate | 40 (%sat) | |
2 | 1 | 1 | sodium acetate | 0.1 (M) | |
3 | 1 | 1 | biotin | 3.8 (mM) |