1DF0
Crystal structure of M-Calpain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F2 |
| Synchrotron site | CHESS |
| Beamline | F2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1998-08-25 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 1 |
| Unit cell lengths | 57.696, 80.181, 80.720 |
| Unit cell angles | 60.37, 70.85, 79.49 |
Refinement procedure
| Resolution | 25.000 - 2.600 |
| R-factor | 0.223 |
| Rwork | 0.223 |
| R-free | 0.29300 |
| RMSD bond length | 0.009 * |
| RMSD bond angle | 1.452 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.041 | 0.110 |
| Number of reflections | 227921 | |
| <I/σ(I)> | 17.1 | |
| Completeness [%] | 97.7 | 98.1 |
| Redundancy | 3.1 | 2.91 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 * | 298 | Hosfield, C.M., (1999) Acta Crystallogr., Sect.D, 55, 1484. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | MES | 100 (mM) | pH6.5 |
| 2 | 1 | drop | PEG6000 | 10-12 (%) | |
| 3 | 1 | drop | 50 (mM) | ||
| 4 | 1 | drop | protein | 10 (mg/ml) |
