1DET

RIBONUCLEASE T1 CARBOXYMETHYLATED AT GLU 58 IN COMPLEX WITH 2'GMP

Experimental procedure

Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Temperature [K]	288
Detector technology	IMAGE PLATE
Collection date	1995-05-16
Detector	RIGAKU
Spacegroup name	I 2 3
Unit cell lengths	88.690, 88.690, 88.690
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 1.800
R-factor	0.194 *
RMSD bond length	0.019
RMSD bond angle	0.045
Data reduction software	WEIS
Refinement software	PROLSQ

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	1.800	1.800 *
Rmerge	0.057
Number of reflections	10459
Completeness [%]	95.9	89.7 *
Redundancy	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	3.2 *	20 *	pH 4.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	2'-GMP	0.5 (%)
3	1	drop	sodium citrate	30 (mM)
4	1	drop	2-methylpentane-2,4-diol	17 (%(v/v))
5	1	reservoir	2-methylpentane-2,4-diol	51 (%(v/v))