1DEL
DEOXYNUCLEOSIDE MONOPHOSPHATE KINASE COMPLEXED WITH DEOXY-GMP AND AMP
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-04-01 |
| Detector | MAR scanner 180 mm plate |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 155.200, 58.500, 75.700 |
| Unit cell angles | 90.00, 108.10, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.200 |
| R-factor | 0.173 * |
| Rwork | 0.186 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 0.027 * |
| Data reduction software | DENZO |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 10.000 |
| High resolution limit [Å] | 2.200 |
| Rmerge | 0.065 |
| Total number of observations | 96619 * |
| Number of reflections | 31836 |
| Completeness [%] | 96.5 |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.6 * | 4 * | pH 6.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 20 (mM) | |
| 3 | 1 | drop | DTT | 7 (mM) | |
| 4 | 1 | drop | dGMP | 5 (mM) | |
| 5 | 1 | reservoir | PEG4000 | 11-14 (%(v/v)) | |
| 6 | 1 | reservoir | magnesium acetate | 200 (mM) | |
| 7 | 1 | reservoir | DTT | 7 (mM) | |
| 8 | 1 | reservoir | dioxane | 2 (%) | |
| 9 | 1 | reservoir | cacodylate | 100 (mM) |
