1DE6
L-RHAMNOSE ISOMERASE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 195 |
Detector technology | IMAGE PLATE |
Detector | RIGAKU RAXIS II |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 169.179, 162.439, 77.726 |
Unit cell angles | 90.00, 109.89, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.100 |
R-factor | 0.173 |
Rwork | 0.244 |
R-free | 0.24400 |
RMSD bond length | 0.019 * |
RMSD bond angle | 2.800 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | TNT |
Refinement software | TNT |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.134 | 0.426 |
Number of reflections | 108398 | |
<I/σ(I)> | 6.8 | |
Completeness [%] | 92.0 | 88 * |
Redundancy | 2 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 4 * | 0.2 M CITRATE, 0.1-M HEPES, 22.5 - 30.0 % PEG 8000, 10 % ISOPROPANOL, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | drop | HEPES | 10 (mM) | |
3 | 1 | drop | 100 (mM) | ||
4 | 1 | reservoir | citrate | 0.2 (M) | |
5 | 1 | reservoir | HEPES | 0.1 (M) | |
6 | 1 | reservoir | PEG8000 | 22.5-30.0 (%(w/w)) | |
7 | 1 | reservoir | isopropanol | 10 (%(w/w)) |