1DE4
HEMOCHROMATOSIS PROTEIN HFE COMPLEXED WITH TRANSFERRIN RECEPTOR
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-1 |
Synchrotron site | SSRL |
Beamline | BL9-1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1999-01-07 |
Detector | MARRESEARCH |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 110.400, 144.400, 327.100 |
Unit cell angles | 90.00, 93.60, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.800 |
R-factor | 0.231 |
Rwork | 0.231 |
R-free | 0.26500 |
Structure solution method | MIRAS |
RMSD bond length | 0.007 |
RMSD bond angle | 23.300 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | CNS (0.5) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.850 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.070 | 0.341 |
Number of reflections | 122846 | |
<I/σ(I)> | 18 | 3.3 |
Completeness [%] | 98.1 | 99.8 |
Redundancy | 2.8 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | PEG 8000, TRIS, TRIMETHYLAMINE HCL, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG8000 | 7-8 (%) | |
2 | 1 | reservoir | Tris-HCl | 200 (mM) | |
3 | 1 | reservoir | trimethylamine HCl | 10 (mM) |