1DDL

DESMODIUM YELLOW MOTTLE TYMOVIRUS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	295
Detector technology	IMAGE PLATE
Collection date	1996-03-28
Detector	MARRESEARCH
Spacegroup name	P 42 3 2
Unit cell lengths	348.546, 348.546, 348.546
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 2.700
R-factor	0.152
Rwork	0.151
R-free	0.15900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	TURNIP YELLOW MOSAIC VIRUS
RMSD bond length	0.010
RMSD bond angle	1.900
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.800
High resolution limit [Å]	2.700	2.700
Rmerge	0.107	0.453
Total number of observations	574527 *
Number of reflections	178705
<I/σ(I)>	7.3
Completeness [%]	91.3 *	82.7
Redundancy	3.2	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	4.8	295	2 M SODIUM FORMATE, 0.1 M SODIUM ACETATE, POTASSIUM PHOSPHATE BUFFER, pH 4.8, VAPOR DIFFUSION, BOTH SITTING DROP AND HANGING DROP, temperature 295K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	SODIUM FORMATE
2	1	1	SODIUM ACETATE
3	1	1	POTASSIUM PHOSPHATE BUFFER
4	1	2	SODIUM FORMATE
5	1	2	SODIUM ACETATE
6	1	2	POTASSIUM PHOSPHATE BUFFER

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	3 (mg/ml)
2	1	reservoir	sodium formate	2.0-2.2 (M)
3	1	reservoir	sodium acetate	0.1 (M)