1DDJ
CRYSTAL STRUCTURE OF HUMAN PLASMINOGEN CATALYTIC DOMAIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-03-15 |
| Detector | BRANDEIS |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 90.490, 46.160, 126.650 |
| Unit cell angles | 90.00, 91.75, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| R-factor | 0.194 |
| Rwork | 0.194 |
| R-free | 0.26700 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.401 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.074 | 0.400 |
| Number of reflections | 58496 | 5533 * |
| <I/σ(I)> | 10.6 | |
| Completeness [%] | 82.1 | 78.4 |
| Redundancy | 2.2 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 6.5 | 20 * | drop solution was mixed 1:1 with the reservoir solution * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | PEG4000 | 12 (%(w/v)) | |
| 3 | 1 | reservoir | 100 (mM) | ||
| 4 | 1 | reservoir | isopropanol | 10 (%(v/v)) | |
| 5 | 1 | reservoir | N-(2-acetamido) iminodiacetic acid | 100 (mM) |
