1DCK
STRUCTURE OF UNPHOSPHORYLATED FIXJ-N COMPLEXED WITH MN2+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-01-01 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 |
| Unit cell lengths | 31.700, 42.500, 44.700 |
| Unit cell angles | 93.20, 103.00, 101.80 |
Refinement procedure
| Resolution | 14.450 - 2.000 |
| R-factor | 0.206 |
| Rwork | 0.206 |
| R-free | 0.25300 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.600 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.051 | 0.196 |
| Total number of observations | 24325 * | |
| Number of reflections | 13062 | |
| <I/σ(I)> | 15.6 | |
| Completeness [%] | 87.0 | 57.7 |
| Redundancy | 1.9 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | PEG1500, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 4K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 12 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 20 (mM) | |
| 3 | 1 | drop | EDTA | 0.1 (mM) | |
| 4 | 1 | drop | dithiothreitol | 1 (mM) | |
| 5 | 1 | reservoir | PEG1500 | 40 (%(w/v)) | |
| 6 | 1 | reservoir | Tris-HCl | 20 (mM) |
