1DBZ
C153S MUTANT OF PEA FRUCTOSE-1,6-BISPHOSPHATASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LURE BEAMLINE D41A |
| Synchrotron site | LURE |
| Beamline | D41A |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-01-01 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 78.900, 114.500, 94.500 |
| Unit cell angles | 90.00, 114.50, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.650 |
| Rwork | 0.235 |
| R-free | 0.29100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | OXIDIZED PEA WILD TYPE FBPASE FORM 1 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.360 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.000 | 2.700 |
| High resolution limit [Å] | 2.600 | 2.650 |
| Rmerge | 0.096 | 0.900 |
| Total number of observations | 291800 * | |
| Number of reflections | 41334 | |
| <I/σ(I)> | 13 | |
| Completeness [%] | 87.6 | 89 |
| Redundancy | 7 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 291 | 20% PEG 1000, 50MM NA ACETATE (PH 5), 50 MM MGCL2, 5MM F6P, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG1000 | 18-20 (%) | |
| 2 | 1 | reservoir | sodium acetate | 50 (mM) | |
| 3 | 1 | reservoir | 50 (mM) | ||
| 4 | 1 | reservoir | F6P | 5 (mM) |
