1DBW
CRYSTAL STRUCTURE OF FIXJ-N
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LURE BEAMLINE DW32 |
| Synchrotron site | LURE |
| Beamline | DW32 |
| Temperature [K] | 100 |
| Detector technology | AREA DETECTOR |
| Collection date | 1998-04-04 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 |
| Unit cell lengths | 31.700, 42.200, 44.600 |
| Unit cell angles | 93.20, 104.50, 101.90 |
Refinement procedure
| Resolution | 14.940 - 1.600 |
| R-factor | 0.187 |
| Rwork | 0.187 |
| R-free | 0.22400 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 24.300 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.071 | 0.123 |
| Total number of observations | 62615 * | |
| Number of reflections | 20628 | |
| <I/σ(I)> | 10 | |
| Completeness [%] | 73.0 | 20.2 |
| Redundancy | 3 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | PEG 1500, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 4K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | PEG 1500, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 4K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 12 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 20 (mM) | |
| 3 | 1 | drop | EDTA | 0.1 (mM) | |
| 4 | 1 | drop | dithiothreitol | 1 (mM) | |
| 5 | 1 | reservoir | PEG1500 | 40 (%(w/v)) | |
| 6 | 1 | reservoir | Tris-HCl | 20 (mM) |
