1DBU
Crystal structure of cysteinyl-tRNA(Pro) deacylase protein from H. influenzae (HI1434)
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1998-10-27 |
| Detector | BRANDEIS |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 102.570, 42.490, 32.540 |
| Unit cell angles | 90.00, 104.17, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.800 |
| Rwork | 0.193 |
| R-free | 0.26200 |
| Structure solution method | MAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 0.032 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.059 | 0.357 |
| Total number of observations | 47185 * | |
| Number of reflections | 25089 | |
| Completeness [%] | 98.7 | 98 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 * | drop consists of equal volume of protein and reservoir solutions * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 6 (mg/ml) | |
| 2 | 1 | drop | potassium phosphate | 50 (mM) | |
| 3 | 1 | drop | EDTA | 0.1 (mM) | |
| 4 | 1 | drop | dithiothreitol | 5 (mM) | |
| 5 | 1 | reservoir | Tris-HCl | 100 (mM) | |
| 6 | 1 | reservoir | mPEG5000 | 25-35 (%) | |
| 7 | 1 | reservoir | propanol | 3 (%) |
