1DB3
E.COLI GDP-MANNOSE 4,6-DEHYDRATASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1998-11-01 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 149.600, 149.600, 102.600 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.300 |
| R-factor | 0.205 |
| Rwork | 0.205 |
| R-free | 0.23200 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.360 |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.076 | 0.526 |
| Total number of observations | 375043 * | |
| Number of reflections | 30549 * | |
| <I/σ(I)> | 29.4 | |
| Completeness [%] | 99.7 | 99.1 |
| Redundancy | 12.3 | 9.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 4 * | SODIUM TARTRATE, HEPES, DTT, SODIUM CHLORIDE, TRIS, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 6 (mg/ml) | |
| 2 | 1 | drop | Na/K tartrate | 0.8 (M) | |
| 3 | 1 | drop | 50 (mM) | ||
| 4 | 1 | drop | HEPES | 50 (mM) | |
| 5 | 1 | drop | Tris-HCl | 10 (mM) | |
| 6 | 1 | drop | dithiothreitol | 1 (mM) | |
| 7 | 1 | reservoir | Na/K tartrate | 0.8 (M) | |
| 8 | 1 | reservoir | HEPES | 100 (mM) |
