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CRYSTAL STRUCTURE OF A BINARY COMPLEX OF PROTEIN KINASE CK2 (ALPHA-SUBUNIT) AND MG-GMPPNP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ENRAF-NONIUS FR571
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-11-10
Detector	MAC Science DIP-2030
Spacegroup name	C 1 2 1
Unit cell lengths	142.700, 58.820, 46.180
Unit cell angles	90.00, 103.77, 90.00

Refinement procedure

Resolution	54.100 - 2.200
R-factor	0.212 *
Rwork	0.212
R-free	0.29000
Starting model (for MR)	1daw
RMSD bond length	0.008 *
RMSD bond angle	2.800 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.100	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.107	0.407
Number of reflections	19002
<I/σ(I)>	12.5
Completeness [%]	99.9	99.2
Redundancy	7.1	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5 *	292 *	Guerra, B., (1998) Acta Crystallogr., Sect.D, 54, 143. *
1	VAPOR DIFFUSION, SITTING DROP	8.5 *	292 *	Guerra, B., (1998) Acta Crystallogr., Sect.D, 54, 143. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	8 (mg/ml)
2	1	drop		500 (mM)
3	1	drop	Tris-HCl	25 (mM)
4	1	drop	2-mercaptoethanol	7 (mM)
5	1	reservoir	ATP	6 (mM)
6	1	reservoir		1.5 (mM)