1DAN
Complex of active site inhibited human blood coagulation factor VIIA with human recombinant soluble tissue factor
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.5 |
| Synchrotron site | SRS |
| Beamline | PX9.5 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 70.650, 82.550, 126.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| R-factor | 0.187 |
| Rwork | 0.187 |
| R-free | 0.21800 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 24.260 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 1.950 |
| Rmerge | 0.076 |
| Total number of observations | 162150 * |
| Number of reflections | 54736 |
| <I/σ(I)> | 10.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | sparse matrix screen * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Sephacryl S-200 column | 10 (mg/ml) |
