1D8X
CRYSTAL STRUCTURE OF DNA SHEARED TANDEM G A BASE PAIRS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 123 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-02-06 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 62.784, 35.685, 22.466 |
| Unit cell angles | 90.00, 104.38, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.200 |
| R-factor | 0.182 * |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.029 |
| Data scaling software | d*TREK (V. 5.1) |
| Phasing software | X-PLOR |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.240 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.045 | 0.390 |
| Number of reflections | 12550 | |
| <I/σ(I)> | 16.5 | 3.7 |
| Completeness [%] | 81.7 | 46.8 |
| Redundancy | 1.6 | 1.25 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 298 | MPD, COBALT HEXAMMINE, MGCL2, TRIS, pH 7.5, VAPOR DIFFUSION, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | COBALT HEXAMMINE | ||
| 2 | 1 | 1 | MGCL2 | ||
| 3 | 1 | 1 | TRIS | ||
| 4 | 1 | 1 | MPD | ||
| 5 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | DNA duplex | 1.3 (mM) | |
| 2 | 1 | drop | Tris-HCl | 2 (mM) | |
| 3 | 1 | drop | 5 (mM) | ||
| 4 | 1 | drop | 5 (mM) | ||
| 5 | 1 | drop | 2.6 (%) | ||
| 6 | 1 | reservoir | MPD | 15 (%) |
