1D7P

Crystal structure of the c2 domain of human factor viii at 1.5 a resolution at 1.5 A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	150
Detector technology	IMAGE PLATE
Collection date	1998-11-22
Detector	RIGAKU RAXIS
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	46.440, 56.410, 65.740
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 1.500
R-factor	0.206
Rwork	0.205
R-free	0.21500
RMSD bond length	0.006
RMSD bond angle	1.470
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.530
High resolution limit [Å]	1.500	1.500
Rmerge	0.040	0.188
Number of reflections	27327
<I/σ(I)>	13.7
Completeness [%]	96.0	93.9
Redundancy	4.5	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	6 *	298	AMMONIUM SULFATE, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K
1	unknown *	6 *	298	AMMONIUM SULFATE, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K
1	unknown *	6 *	298	AMMONIUM SULFATE, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K
1	unknown *	6 *	298	AMMONIUM SULFATE, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	ammonium sulfate	2 (M)
2	1	1	MES	0.1 (M)
3	1	1		0.015 (%)