1D7M

COILED-COIL DIMERIZATION DOMAIN FROM CORTEXILLIN I

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7B
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7B
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-11-14
Detector	MARRESEARCH
Spacegroup name	C 2 2 21
Unit cell lengths	70.420, 128.650, 91.570
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 * - 2.700
R-factor	0.218 *
Rwork	0.205
R-free	0.25700 *
RMSD bond length	0.170 *
RMSD bond angle	1.770 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.850
High resolution limit [Å]	2.700	2.700
Rmerge	0.078 *	0.462
Total number of observations	49645 *
Number of reflections	11302
<I/σ(I)>	5.3
Completeness [%]	94.6 *	88.2
Redundancy	2.8	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4 *	297	Burkhard, P., (1998) J.Struct.Biol., 122, 293. *
1	VAPOR DIFFUSION, HANGING DROP	7.4 *	297	Burkhard, P., (1998) J.Struct.Biol., 122, 293. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	sodium phosphate	5 (mM)
2	1	drop	protein	15 (mg/ml)
3	1	drop		150 (mM)
4	1	reservoir	ammonium sulfate	1.5 (M)
5	1	reservoir	Tris	0.1 (M)
6	1	reservoir	PEG400	2.5 (%)
7	1	reservoir	dioxane	1 (%)