1D5T
GUANINE NUCLEOTIDE DISSOCIATION INHIBITOR, ALPHA-ISOFORM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 97 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-02-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 89.290, 42.740, 61.780 |
| Unit cell angles | 90.00, 104.55, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.040 |
| R-factor | 0.173 |
| R-free | 0.20900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gnd |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.030 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 1.070 |
| High resolution limit [Å] | 1.040 | 1.040 |
| Rmerge | 0.071 | 0.500 |
| Number of reflections | 207147 | |
| <I/σ(I)> | 5.3 | |
| Completeness [%] | 96.0 | 97 |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.25 * | 295.5 | ammonium sulfate, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 22.5K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ammonium sulfate | 1.73 (M) |
