1D4T
CRYSTAL STRUCTURE OF THE XLP PROTEIN SAP IN COMPLEX WITH A SLAM PEPTIDE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | PRINCETON 2K |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 25.870, 42.910, 44.950 |
| Unit cell angles | 90.00, 98.26, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.100 |
| R-factor | 0.13 |
| Rwork | 0.115 |
| R-free | 0.16500 |
| Structure solution method | AB INITIO |
| RMSD bond length | 0.017 * |
| RMSD bond angle | 0.032 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 22.000 |
| High resolution limit [Å] | 1.100 * |
| Rmerge | 0.043 |
| Total number of observations | 134878 * |
| Number of reflections | 34081 * |
| Completeness [%] | 86.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 295 | 30% PEG 8000, 20% GLYCEROL, 100 MM HEPES PH 8.0, AND 100 MM DTT, VAPOR DIFFUSION, HANGING DROP, temperature 22K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | HEPES | 20 (mM) | |
| 3 | 1 | drop | 150 (mM) | ||
| 4 | 1 | drop | dithiothreitol | 10 (mM) | |
| 5 | 1 | reservoir | PEG8000 | 30 (%) | |
| 6 | 1 | reservoir | glycerol | 20 (%) | |
| 7 | 1 | reservoir | HEPES | 100 (mM) | |
| 8 | 1 | reservoir | dithiothreitol | 10 (mM) |
