1D41
STABILIZATION OF Z-DNA BY DEMETHYLATION OF THYMINE BASES: 1.3 ANGSTROMS SINGLE-CRYSTAL STRUCTURE OF D(M5CGUAM5CG)
Experimental procedure
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 17.820, 30.440, 44.520 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | ? - 1.300 |
| R-factor | 0.208 |
| Starting model (for MR) | ZDFB06 IN WHICH THE METHYL GROUPS OF THE THYMINE BASES WERE REMOVED FROM THE C5 POSITIONS. |
| Refinement software | NUCLSQ |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 1.300 |
| Number of reflections | 2870 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | pH 7.00, VAPOR DIFFUSION |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | NA CACODYLATE | ||
| 4 | 1 | 1 | MGCL2 | ||
| 5 | 1 | 2 | WATER | ||
| 6 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | MPD | 30 (%) | |
| 2 | 1 | drop | oligonucleotide | 4.0 (mM) | |
| 3 | 1 | drop | sodium cacodylate | 28 (mM) | |
| 4 | 1 | drop | magnesium chloride | 15 (mM) | |
| 5 | 1 | drop | MPD | 8.5 (%) |
