1D3Y
STRUCTURE OF THE DNA TOPOISOMERASE VI A SUBUNIT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.510, 59.040, 87.260 |
| Unit cell angles | 90.00, 94.05, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| R-factor | 0.197 * |
| Rwork | 0.197 |
| R-free | 0.24200 |
| RMSD bond length | 0.006 * |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.036 | 0.131 |
| Number of reflections | 43773 | |
| <I/σ(I)> | 17.8 | |
| Completeness [%] | 96.5 | 93.6 |
| Redundancy | 8.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 * | 20 * | PEG 8000, MPD, NA-CACODYLATE, MGCL2, NACL, pH 6.1, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 10 (mM) | |
| 3 | 1 | drop | 200 (mM) | ||
| 4 | 1 | reservoir | MPD | 20-25 (%) | |
| 5 | 1 | reservoir | PEG3000 | 13-16 (%) | |
| 6 | 1 | reservoir | 400-425 (mM) | ||
| 7 | 1 | reservoir | 160-200 (mM) | ||
| 8 | 1 | reservoir | sodium cacodylate-HCl | 50 (mM) |
