1D3W

Crystal structure of ferredoxin 1 d15e mutant from azotobacter vinelandii at 1.7 angstrom resolution.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1999-07-09
Detector	RIGAKU RAXIS IV
Spacegroup name	P 41 21 2
Unit cell lengths	55.370, 55.370, 92.535
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 1.700
R-factor	0.209
Rwork	0.209
R-free	0.24700
RMSD bond length	0.006
RMSD bond angle	1.895
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	1.720
High resolution limit [Å]	1.700	1.700
Rmerge	0.080	0.546
Number of reflections	16030
<I/σ(I)>	17.38
Completeness [%]	96.5	89.5
Redundancy	13.81

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.8	277	Stout, C.D., (1989 J. Mol. Biol., 205, 545. *