1D3U

TATA-BINDING PROTEIN/TRANSCRIPTION FACTOR (II)B/BRE+TATA-BOX COMPLEX FROM PYROCOCCUS WOESEI

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	1998-12-08
Detector	CUSTOM-MADE
Spacegroup name	I 21 3
Unit cell lengths	174.200, 174.200, 174.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.400
R-factor	0.212
Rwork	0.206
R-free	0.24500
RMSD bond length	0.012
RMSD bond angle	1.600
Data reduction software	HKL-2000
Data scaling software	SCALA
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.300	2.530
High resolution limit [Å]	2.400	2.400
Rmerge	0.068	0.339
Number of reflections	33982
<I/σ(I)>	7.3
Completeness [%]	99.2	99.2
Redundancy	5.2	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8 *	19 *	PEG 400, STRONTIUM CHLORIDE, SODIUM CITRATE, TRIS, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	SRCL2
2	1	1	SODIUM CITRATE
3	1	1	TRIS
4	1	1	PEG 400
5	1	2	PEG 400

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	TBP	0.4 (mM)
2	1	drop	TFB	0.4 (mM)
3	1	drop	DNA	0.6 (mM)
4	1	drop	potassium acetate	200 (mM)
5	1	drop	HEPES	50 (mM)
6	1	reservoir	PEG400	30 (%(w/v))
7	1	reservoir	SrCl2	25 (mM)
8	1	reservoir	sodium citrate	200 (mM)
9	1	reservoir	Tris	50 (mM)