1D2K
C. IMMITIS CHITINASE 1 AT 2.2 ANGSTROMS RESOLUTION
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 298 |
Detector technology | IMAGE PLATE |
Collection date | 1997-05-16 |
Detector | RIGAKU RAXIS IV |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 91.248, 91.248, 95.400 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 5.000 - 2.200 |
R-factor | 0.178 |
Rwork | 0.170 |
R-free | 0.25800 * |
RMSD bond length | 0.007 * |
RMSD bond angle | 1.360 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.122 | 0.213 |
Number of reflections | 20899 | |
<I/σ(I)> | 10.3 | |
Completeness [%] | 99.7 | 99.4 |
Redundancy | 6 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 298 | PEG 4000, SODIUM ACETATE, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 25K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 20 (mg/ml) | |
2 | 1 | drop | chitinase | 4 (mg/ml) | |
3 | 1 | reservoir | PEG4000 | 30 (%) | |
4 | 1 | reservoir | sodium acetate | 0.1 (M) |