1D2I

CRYSTAL STRUCTURE OF RESTRICTION ENDONUCLEASE BGLII COMPLEXED WITH DNA 16-MER

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	123
Detector technology	CCD
Collection date	1999-02-28
Detector	MARRESEARCH
Wavelength(s)	0.9494, 0.9791, 0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	48.800, 101.700, 116.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.700
R-factor	0.182
Rwork	0.182
R-free	0.20000
RMSD bond length	0.018
RMSD bond angle	1.880 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	99.000	1.780
High resolution limit [Å]	1.700	1.700
Rmerge	0.069 *	0.162
Total number of observations	629080 *
Number of reflections	123664
<I/σ(I)>	13
Completeness [%]	99.8	99.3
Redundancy	3.8	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Batch method *	5.2	20 *	15- 20% PEG 4000, 0.2 M AMMONIUM SULFATE, 0.1 M MES PH 5.2, MICROBATCH, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	8-10 (mg/ml)
2	1	1	PEG1540	17-20 (%)
3	1	1	ammonium sulfate	0.2 (M)
4	1	1	MES	0.1 (M)