1D2G

CRYSTAL STRUCTURE OF R175K MUTANT GLYCINE N-METHYLTRANSFERASE FROM RAT LIVER

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	1997-01-10
Detector	MACSCIENCE DIP100
Spacegroup name	P 21 21 2
Unit cell lengths	86.700, 176.000, 45.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.500
R-factor	0.194
Rwork	0.194
R-free	0.26600
RMSD bond length	0.007
RMSD bond angle	28.200 *
Data reduction software	ELMS
Data scaling software	KUAVST
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	10.000	2.600
High resolution limit [Å]	2.500	2.500
Rmerge	0.071	0.170
Total number of observations	71872 *
Number of reflections	22390
<I/σ(I)>	3.35
Completeness [%]	91.3	85
Redundancy	3.3	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	277	PEG-4000, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	sodium citrate	100 (mM)
2	1	drop	ammonium acetate	100 (mM)
3	1	drop		50 (mM)
4	1	drop	PEG4000	13 (%(w/v))
5	1	drop	protain	10 (mg/ml)