1D2C
METHYLTRANSFERASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-11-11 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 86.300, 175.900, 45.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.500 |
| R-factor | 0.175 |
| Rwork | 0.175 |
| R-free | 0.23300 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 27.800 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.069 | 0.980 |
| Total number of observations | 69713 * | |
| Number of reflections | 22488 | |
| <I/σ(I)> | 3.15 | |
| Completeness [%] | 92.2 | 92.2 |
| Redundancy | 3.1 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5.6 | 277 | PEG 3400, pH 5.6, VAPOR DIFFUSION, temperature 4.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | sodium citrate | 100 (mM) | |
| 2 | 1 | drop | ammonium acetate | 100 (mM) | |
| 3 | 1 | drop | 50 (mM) | ||
| 4 | 1 | drop | PEG4000 | 13 (%(w/v)) | |
| 5 | 1 | drop | protain | 10 (mg/ml) |
