1D1Q

CRYSTAL STRUCTURE OF A YEAST LOW MOLECULAR WEIGHT PROTEIN TYROSINE PHOSPHATASE (LTP1) COMPLEXED WITH THE SUBSTRATE PNPP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	123
Detector technology	IMAGE PLATE
Collection date	1998-03-10
Detector	RIGAKU RAXIS IV
Spacegroup name	P 21 21 21
Unit cell lengths	45.897, 64.151, 112.049
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	12.000 - 1.700
R-factor	0.17
Rwork	0.170
R-free	0.21500
RMSD bond length	0.013
RMSD bond angle	23.700 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.760
High resolution limit [Å]	1.700	1.700
Rmerge	0.062	0.242
Total number of observations	240634 *
Number of reflections	32773
<I/σ(I)>	24.9
Completeness [%]	87.8	50.2
Redundancy	7.3	1.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	PEG 3400, Bis-TRIS, sodium chloride para-nitrophenyl phosphate, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293.0K