1CZW
STRUCTURE OF THE W34A MUTANT OF SHIGA-LIKE TOXIN I B SUBUNIT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | SIEMENS |
| Temperature [K] | 298 |
| Detector technology | AREA DETECTOR |
| Collection date | 1995-04-22 |
| Detector | SIEMENS |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 107.550, 108.920, 57.350 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.600 - 2.500 |
| R-factor | 0.182 |
| Rwork | 0.182 |
| R-free | 0.21400 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.967 |
| Data reduction software | X-GEN |
| Data scaling software | X-GEN |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.600 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.127 | 0.365 |
| Number of reflections | 23240 | |
| <I/σ(I)> | 11.5 | |
| Completeness [%] | 96.8 | 53.3 |
| Redundancy | 6.7 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 15% PEG 4000 0.2 M MGCL2 5% DIOXANE, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
