1CZV

CRYSTAL STRUCTURE OF THE C2 DOMAIN OF HUMAN COAGULATION FACTOR V: DIMERIC CRYSTAL FORM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	289
Detector technology	IMAGE PLATE
Collection date	1998-06-07
Detector	MARRESEARCH
Spacegroup name	P 21 21 2
Unit cell lengths	86.520, 70.540, 60.580
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.400
R-factor	0.205
Rwork	0.205
R-free	0.25700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1czs
RMSD bond length	0.006
RMSD bond angle	1.390
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.600	2.510
High resolution limit [Å]	2.400	2.400
Rmerge	0.094	0.399
Number of reflections	14635
Completeness [%]	96.8	90.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	10	20 *	pH 10.00

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	MOPS	5 (mM)	pH8.0
2	1	drop	protein	6 (mg/ml)
3	1	reservoir	potassium phosphate	0.1 (M)
4	1	reservoir	ammonium sulfate	0.7 (M)	pH9.5-10.0