1CZ7
THE CRYSTAL STRUCTURE OF A MINUS-END DIRECTED MICROTUBULE MOTOR PROTEIN NCD REVEALS VARIABLE DIMER CONFORMATIONS
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1998-12-20 |
Detector | MARRESEARCH |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 116.194, 148.827, 261.516 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.000 - 2.900 |
R-factor | 0.258 |
Rwork | 0.258 |
R-free | 0.29400 |
RMSD bond length | 0.014 * |
RMSD bond angle | 1.600 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.085 | 0.280 * |
Total number of observations | 136320 * | |
Number of reflections | 48773 | |
Completeness [%] | 96.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.8 * | 20 * | PEG 4000, NACL, MGCL2, K3PO4, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 5 (mg/ml) | |
10 | 1 | reservoir | 2 (M) | ||
11 | 1 | reservoir | dithiothreitol | 7 (mM) | |
2 | 1 | drop | PEG8000 | 6.8 (%) | |
3 | 1 | drop | 1 (M) | ||
4 | 1 | drop | ATP | 2 (mM) | |
5 | 1 | drop | dithiothreitol | 3.5 (mM) | |
6 | 1 | drop | 10 (mM) | ||
7 | 1 | drop | sodium phosphate | 25 (mM) | |
8 | 1 | reservoir | sodium phophate | 25 (mM) | |
9 | 1 | reservoir | PEG8000 | 13.5 (%) |